Biomolecular Interactions

Unique applications for Biomolecular Interactions using real-time LSPR

Insplorion’s NPS technology is an effective method for real-time in-situ studies of kinetic processes at molecular interfaces. The technique is label free, extremely surface sensitive and compatible with both thin- and thick films. Insplorion’s sensors consist of an array of gold nanodisks on a glass plate. Temperature can be varied very flexibly and the sensors can be coated with a variety of chemistries depending on what the Insplorion instrument should measure. If you have a challenge where our technology might help you, contact Patrik Bjöörn, our applications engineer.

Different sensor coatings are available for tailored surface chemistry and/or linking chemistry. Standard sensor chemistry: SiO2, Si3N4, TiO2 and Al2O3 allows for modification with for example , SAMs, Silanes, Polymers. Custom coatings are available on request, please contact Patrik Bjöörn.

Drug Delivery

Insplorion NPS can be used to monitor diffusion in polymer films with thickness ranging from microns to a few nanometer as well as porous networks. The Insplorion sensor, with its extreme surface sensitivity, probes the hidden internal interface in thick films. This allows you to determine the time for a diffusing species to reach the interface and saturate the film, as well as monitoring its realease. Quantitative kinetic information such as the diffusion coefficient has in one case been derived from the experiment.

Monitoring Lipid Bilayers and Vesicles

Insplorion’s technology and instrument allows for complete experiments wherein both lipid bilayer formation and interactions with biomolecules and nanoparticles can be monitored. The extreme sensitivity to optical property changes near the surface also enables structural information to be obtained, for example on shape of vesicles.

Molecular Binding and Biorecognition

Insplorion NPS is suitable for biomolecular interaction analysis. By monitoring the immobilization of a capturing agent (ligand) to the Insplorion sensor and then introduce the analyte via the fluid system of the Insplorion instrument, quantitative information, as affinity constants, can be determined.